Officers received a report of the two vehicle collision at 2:51 pm Wednesday at highway 21 and Kincardine Avenue.
They, Kincardine Fire & Bruce County EMS arrived and both the motorcycle driver & passenger were airlifted to a London hospital.
The driver in serious, non-life threatening condition and the passenger with life-threatening injuries.
The pickup driver went to local hospital for treatment.
The intersection was closed for protection of emergency responders but has since reopened.
The OPP Traffic Management & Enforcement Unit are assisting in the investigation with the investigation in its early stages.